Evaluation of penetration depth of 2% chlorhexidine digluconate into root dentinal tubules using confocal laser scanning microscope
Vadhana Sekar, Velmurugan Jothi Latha and Natanasabapathy,
소속 상세정보
( Vadhana Sekar ) - Meenakshi Ammal Dental College and Hospital Department of Conservative Dentistry and Endodontics
( Velmurugan Jothi Latha and Natanasabapathy ) - Meenakshi Ammal Dental College and Hospital Department of Conservative Dentistry and Endodontics
KMID : 1034420150400020149
Abstract
Objectives: This study evaluated the penetration depth of 2% chlorhexidine digluconate (CHX) into root dentinal tubules and the influence of passive ultrasonic irrigation (PUI) using a confocal laser scanning microscope (CLSM).
Materials and Methods: Twenty freshly extracted anterior teeth were decoronated and instrumented using Mtwo rotary files up to size 40, 4% taper. The samples were randomly divided into two groups (n = 10), that is, conventional syringe irrigation (CSI) and PUI. CHX was mixed with Rhodamine B dye and was used as the final irrigant. The teeth were sectioned at coronal, middle and apical levels and viewed under CLSM to record the penetration depth of CHX. The data were statistically analyzed using Kruskal-Wallis and Mann-Whitney U tests.
Results: The mean penetration depths of 2% CHX in coronal, middle and apical thirds were 138 μm, 80 μm and 44 μm in CSI group, respectively, whereas the mean penetration depths were 209 μm, 138 μm and 72 μm respectively in PUI group. Statistically significant difference was present between CSI group and PUI group at all three levels (p < 0.01 for coronal third and p < 0.001 for middle and apical thirds). On intragroup analysis, both groups showed statistically significant difference among three levels (p < 0.001).
Conclusions: Penetration depth of 2% CHX into root dentinal tubules is deeper in coronal third when compared to middle and apical third. PUI aided in deeper penetration of 2% CHX into dentinal tubules when compared to conventional syringe irrigation at all three levels.
키워드
Chlorhexidine digluconate; Passive ultrasonic irrigation; Penetration depth; Root dentinal tubules
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